TRPA1 is involved in visceral spasms.

(A) CFU counting in the midgut of WT (grey bars) or TrpA11 homozygote (blue bars) flies fed with 108 CFU of Btk. (B) Measure of midgut length of TrpA11 homozygote flies 2 h post-intoxication with sucrose (Ctrl) or 108 CFU of Btk. (C) Phalloidin staining of posterior midgut of TrpA11 homozygote flies 2 h after feeding with 5% sucrose (Ctrl) or 108 CFU of Btk. 20X objective. (D) Upper picture: reconstructed image of a WT midgut (10X objective). The midgut was stained for DH31 (Green) and Prospero (Red, Pros) that marks all the EECs. DAPI (blue) marks the nuclei. Anterior is to the left and posterior to the right. Lower insets: magnifications (40X objective) of the R2a (left), R2b (middle) and R4c (right) regions (http://flygut.epfl.ch/). (E-G and I-K) R2b anterior midgut region of WT flies (E and I-K) and TrpA11 homozygote flies (F and G) fed either with sucrose (E and F) or with 108 CFU of Btk (G and I-K). (G and I-K) midguts were dissected and fixed 15 min, 30 min and 1 h PI as indicated on the pictures. Bright green arrows point EECs with a strong DH31 labelling and dark green arrows point EECs with a lower labelling. (H) Counting of high intensity DH31-positive EECs in the anterior R2b domain in conditions described in (E-G). (L) Counting of all DH31-positive EECs in the anterior R2b domain in conditions described in (I-K). (M and N) Fast AiryScan imaging of one representative EEC in the anterior R2b midgut domain of WT flies 1h PI of sucrose (M) or 108 CFU of Btk (N).