Additional file 5: of Evaluation of anti-PD-1-based therapy against triple-negative breast cancer patient-derived xenograft tumors engrafted in humanized mouse models

a Evaluation of the percentages of human CD45+ TILs present in MC1 PDX tumors engrafted in hNSG mice and collected from animals treated with either vehicle control or anti-PD-1 antibody. The values represent the mean ± SEM (n = 8). b Schematic representation of the method used to determine the cytotoxic activity of TILs by measuring the levels of the lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon TIL-induced tumor cell lysis. TILs were isolated from MC1 PDX tumors engrafted in hNSG mice and treated with either vehicle or anti-PD1 antibody that were cocultured with disaggregated MC1 tumor cells obtained from the corresponding PDX grown in nonhumanized NSG mice. (PPTX 131 kb