Selection of iPSC line SR1423. (A) Selection scheme. (B) SR1423 expression of endoderm markers Sox17 (red) and HNF3ß (green) on Day 3 of differentiation. (C) SR1423 expression of pancreatic markers Pdx1 (red) and Nkx6.1 (green) on Day 11 of differentiation. “Merge” images include nuclear stain (blue). Scale bars are 100 or 200 μm, as indicated.</p
<p>(A–B) QPCR expression analysis of markers representing pancreatic progenitors and mature pancreat...
<p>Immunostaining for NKX6-1 (green) and SOX9 (red) was carried out on day 12 in parent CyT49 hESCs ...
<p>(A) hESCs seeded at different densities were differentiated for 14 days and immunostained for PDX...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
SR1423 cells differentiate poorly to the mesodermal lineage. (A) Positive control is an iPSC line th...
<p>A) Protocol time-line for pancreatic endocrine differentiation from iENDO cells. B) Morphology of...
<p>A–B: DE at stage II; C–D: Pancreatic progenitors at stage III; E-F: Pancreatic progenitors at sta...
Figure S1. Expression of endodermal markers in hPSC-derived definitive endoderm (DE). (A) Immunofluo...
<p>A) Cells were induced to differentiate through progression of stages 1 and 2 with or without DM a...
The generation of pancreatic endocrine and exocrine functional precursors from embryonic stem cells ...
<p>A) Expression of CD45 was compared for SP (green line) and non-SP (black dotted line) cells. (B–H...
<p>(A) At Day 14, the differentiated hESCs were positively stained with antibodies against glucagon....
To isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the exp...
<p>Representative confocal images of Human Pancreatic Carcinoma (PANC-1) cells are shown in rows. Nu...
<p>A–C: An embroid body (EB) at stage II; D: Pancreatic progenitors at stage III; F-G: Pancreatic pr...
<p>(A–B) QPCR expression analysis of markers representing pancreatic progenitors and mature pancreat...
<p>Immunostaining for NKX6-1 (green) and SOX9 (red) was carried out on day 12 in parent CyT49 hESCs ...
<p>(A) hESCs seeded at different densities were differentiated for 14 days and immunostained for PDX...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
SR1423 cells differentiate poorly to the mesodermal lineage. (A) Positive control is an iPSC line th...
<p>A) Protocol time-line for pancreatic endocrine differentiation from iENDO cells. B) Morphology of...
<p>A–B: DE at stage II; C–D: Pancreatic progenitors at stage III; E-F: Pancreatic progenitors at sta...
Figure S1. Expression of endodermal markers in hPSC-derived definitive endoderm (DE). (A) Immunofluo...
<p>A) Cells were induced to differentiate through progression of stages 1 and 2 with or without DM a...
The generation of pancreatic endocrine and exocrine functional precursors from embryonic stem cells ...
<p>A) Expression of CD45 was compared for SP (green line) and non-SP (black dotted line) cells. (B–H...
<p>(A) At Day 14, the differentiated hESCs were positively stained with antibodies against glucagon....
To isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the exp...
<p>Representative confocal images of Human Pancreatic Carcinoma (PANC-1) cells are shown in rows. Nu...
<p>A–C: An embroid body (EB) at stage II; D: Pancreatic progenitors at stage III; F-G: Pancreatic pr...
<p>(A–B) QPCR expression analysis of markers representing pancreatic progenitors and mature pancreat...
<p>Immunostaining for NKX6-1 (green) and SOX9 (red) was carried out on day 12 in parent CyT49 hESCs ...
<p>(A) hESCs seeded at different densities were differentiated for 14 days and immunostained for PDX...