Paq treatment reduces gene expression of TGF-β, CCR2 and MMP2.

Quantitative PCR analysis of gene expression in total liver lysate demonstrating a change in mRNA of (A) TGF-β, (B) CCR2 and (C) MMP2 in N-IF mice treated with Paquinimod (n = 3) or with vehicle (n = 4) for 4 weeks relative to 24αβNOD mice treated with vehicle for 4 weeks (n = 4). (D) Change in mRNA of TGF-β in N-IF mice treated with Paquinimod (n = 4) or with vehicle (n = 4) for 10 weeks relative to 24αβNOD mice (n = 4). The graphs show box plots and the horizontal line represents median value. Pooled data from two independent experiments are shown. n.s. = not significant; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, unpaired t-test.