Pea3 induces both the feedback and feedforward circuits in FGF signaling.

(A) The heatmap of Pea3-dependent genes involved in feedforward and feedback regulation of FGF signaling. P: promoter region, E: enhancer region. (B) Analysis of ChIP-seq data in the LoVo and GIST48 cancer cells line showed binding sites for the Pea3 transcription factors in the promoter and enhancer regions of Pea3, Dusp6 and Col2a1. (C) The Pea3-dependent genes confirmed by RNA in situ hybridization and immunostaining in the lacrimal gland bud (dotted lines). Arrows indicate the basement membrane staining of Col2a1. Scale bars, 50μm.