Cell cycle and apoptosis of ASCs and CG5 with PTX treatments and preliminary experiment on the therapeutic efficacy of PTX-primed ASCs on tumor growth <i>in vivo</i>.

A) Cell cycle analysis by flow cytometry of adherent and not adherent cells processed for PI staining. B) Cytofluorimetric bi-parametric analysis of the annexin V versus PI staining assay. The percentage reported in the annexin V+/PI+ region of each histogram represents the apoptotic cells. C) preliminary study was performed on nude mice (5 mice/group) injected intramuscularly (i.m.) with CG5 cells alone (3x106) or mixed, at ratio of 2:1, with ASCs (1.5x106) unloaded or primed with PTX (2μM) for 6 hours; tumor growth was reported. t-test. (TIF)