Additional file 3: of Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state

Figure S3. RAW264.7 macrophage expression of TNF-α and PGE2 in response to MSU crystals. RAW264.7 macrophages were cultured with or without 0.5 mg/mL MSU crystals for 24 h for preparation of MSU crystal-stimulated conditioned medium and control conditioned medium, respectively. The concentration of TNF-α, PGE2, IL-1β, IL-6, RANKL, and OPG in conditioned medium samples were measured by ELISA. IL-6 and RANKL were undetected in all samples. Data shown are pooled from three biological repeats and are presented as mean (SEM), two-tailed paired t test as indicated between groups. (JPG 156 kb