Gal-9 epitope recognition by Gal-Nab1 and Gal-Nab2.

A. Apoptotic cell death assessed by flow cytometry in CD3+ T cells treated for 36h with gal-9 (gal-9S; 40 nM) alone or in combination with mAbs (ctrl IgG1, Gal-Nab1, Gal-Nab2; 67 nM) pre-incubated or not with scramble peptide (Scr.) or gal-9 CTB-peptide (6.7 μM). B. Human recombinant gal-9 was immobilized in 96-wells plates and binding of Gal-Nab1 and Gal-Nab2 mAbs was measured after pre-incubation with overlapping peptides representative of human gal-9, as described under “Material and Methods”. Gal-Nab1 (left) or Gal-Nab2 (right) were then detected using secondary HRP-conjugated anti-mouse antibodies. Percentages of inhibition induced by each peptide were calculated from the absorbance data as described under “Material and Methods”.