Additional file 5: of Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells

Figure S5. NgBR knockdown attenuated EGF-stimulated signaling and ERα phosphorylation in T47D-TamR cells. (A) T47D-TamR cells were transfected with siNgBR and treated with EGF (100 ng/mL) for 5 min. Downstream signaling of the EGF pathway was determined using western blot assay. (B) Quantitative analysis of phosphorylated proteins presented in Additional file 10: Figure S6A were carried out using ImageJ and were normalized to total proteins. The data are from three separate repeated experiments and are presented as the mean ± SD (*p < 0.05, n = 3). (PDF 310 kb