The change of cell arrangement, GAG synthesis, cartilage surface marker expression and collagen fiber arrangement by stimulation.

GAG expression was confirmed by Safranin-O staining. Stimulated tissues were express more amount of GAG. Scale bar is 400 μm. Lubricin (superficial zone protein) expression was confirmed by immunohistochemistry. Scale bar is 400 μm. In the stimuli group, lubricin expression was intensely expressed in the surface of tissue. Total collagen fiber arrangement was confirmed by Sirius Red staining. In the stimuli group, total collagen fiber was expressed intensely and make horizontal with the surface. But, static group had shown that collagen was weakly. Scale bar is 100 μm.