Immunolocalization of LmjF.21.0725 in <i>L</i>. <i>major</i>.

(A) Subcellular localization of Lmj21.0725 in L. major strains: (+/+: WT; +/PAC/ (+): heterozygous cell line with addback pxNeoGFP-LmjF21.0725; PAC/HYG/ (+): Null mutant with addback pxNeoGFP-LmjF21.0725; Representative cells were visualized with bright field (B/F), DAPI and anti-LmjF.21.0725 antibody overlays. Nuclei and kinetoplasts were stained with DAPI (blue). (B) Western blot analysis with anti-LmjF.21.0725 antibody confirming expression of ~42 kDa LmjF.21.0725 in L. major promastigotes (+/+: WT; +/PAC/ (+): heterozygous cell line with addback pxNeoGFP-LmjF21.0725; HYG/PAC(+): Null mutant with addback pxNeoGFP-LmjF.21.0725; +/PAC/ (V) L. major carrying Leishmania expression vector pXNeoGFP. (C) Growth curve of L. major WT and mutant promastigotes (+/+: WT; +/PAC/ (+): heterozygous cell line with addback pxNeoGFP-LmjF21.0725; HYG/PAC(+): Null mutant with addback pxNeoGFP-LmjF.21.0725; +/PAC/ (V) L. major carrying Leishmania expression vector pXNeoGFP.