Anti-cancer effects of disulfiram in head and neck squamous cell carcinoma via autophagic cell death - Fig 7

A and B. Xenograft experiment. Mice bearing FaDu tumors were treated with DSF/Cu (50 mg/kg/day) 5 days per week for 2 weeks and tumor growth was measured in the control and experimental groups. Tumor growth in the experimental group was significantly less than that of the control group. *p<0.05. C. Tumor tissues was tested by Western blot analysis. Akt signaling was suppressed in DSF treated group and LC3B I/II were significantly increased after DSF treated group. (CTL: control, DSF: DSF treated group) D. Tumor tissues were removed and stained with DAPI and LC3 antibody. Compared to control group (CTL), nuclear degradation was observed only in the experimental group (DSF) and the tumor tissues were strongly stained with LC3 only in the experimental group (DSF).