<i>In vivo</i> conversion of purified mouse astrocytes transduced with SSFV-Sox10-IRES-GFP lentiviral particles after transplantation into the corpora callosa of demyelinated mice brains.

A) Schematic diagram of the experimental procedure of demyelination induction, Y maze test for data presented in Fig 1D and 1E, astrocyte transduction, and transplantation. B) Immunohistofluorescence (IHF) staining against Gfap showed the lack of notable co-expressions of GFP and Gfap as astrocyte marker at 3 weeks post injection (wpi). C-D) Expressions of PdgfRα (C) and NG2 (D) markers in the main population of GFP+ cells in the corpus callosum at 3 wpi. The bottom panels show higher magnifications. E-F) IHF showed that transplanted astrocytes (GFP+) could express mature oligodendrocyte markers Mbp (E) and Plp (F) at 3 wpi. n = 3 animals, CysA: cyclosporine A for preventing rejection of transplanted cells, Scale bars: 50 μm.