Additional file 7: of Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Figure S6. 18S rRNA Nucleotide alignment showing conservation of primer binding sites but not restriction enzyme cut sites in assorted clinically relevant fungi. Although primer binding sites are conserved in all fungal DNA sequences and the XmaI and BamHI restriction enzyme cut sites are present in the human sequence, neither cut site is found in any fungal organism tested, indicating this method may also have increased sensitivity for detecting fungi in eukaryotic hosts. (TIF 18499 kb