MOESM7 of Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II

Additional file 7. PSaV infection is pH-dependent and involves actin and microtubules. LLC-PK cells were either mock-treated or chemical-treated and then infected with PSaV Cowden strain. The cells were then stained with an antibody against the PSaV VPg protein and the number of virus-positive cells was counted by confocal microscopy. Results are shown as the percentages to the number of positive cells in the DMSO vehicle-treated control. All experiments were performed in triplicate. Data are presented as mean Âą standard deviation of the mean from three independent experiments. Differences were evaluated using the one-way ANOVA. *P < 0.05; **P < 0.001; ***P < 0.0001