Additional file 3: of Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Figure S3. Skewed results for W. bancrofti and L. loa due to sample composition. W. bancrofti samples had been collected into vials lacking anticoagulant. Uneven distribution of microfilariae in the clotted samples led to variations in parasite DNA concentrations in each aliquot and inconsistent resultant reads (a). Nevertheless, reductions in human reads per thousand were consistent with other analyses at 1.5- to 2.5-fold (b) despite wide variations in parasite relative reads per thousand (c). Meanwhile, L. loa samples were provided as worm DNA. Because of the lack of human DNA background, L. loa reads per thousand were consistently high (d), and relative reads indicated no fold-change between undigested and digested samples (e). Data shown represents results for 3 biological replicate runs. (TIF 6651 kb