Translocation of transcription factors p-CREB and p-c-Jun into the nuclei of colon epithelial cells during <i>T</i>. <i>cruzi</i> infection.

Primary HCoEpiC grown on Lab-Tek chamber slides were challenged with T. cruzi at multiple time points, washed, fixed, perforated with 0.1% Triton-X100, blocked with 3% BSA-PBS, and incubated at 4°C overnight in solutions containing phalloidin and one of the following antibodies: (A) anti-human p-CREB-Alexa 647, (C) anti-human-p-c-JunS73-Alexa 488. The slides were washed and mounted with mounting media containing DAPI to stain the nuclei. Stained slides were analyzed by confocal microscopy and the mean fluorescence intensity (MFI) values were plotted for (B) p-CREB, (D) p-c-JunS73. Scale bar, 20 μm. Each confocal microscopy image is a representative of three independent biological replicates. The bar graphs represent MFI values ± SE from three independent biological replicates. The value of p<0.05 was considered significant. *p<0.05; **p<0.001.