Additional file 1: of The piRNA pathway responds to environmental signals to establish intergenerational adaptation to stress

Figure S1. Change in 22G small RNA from other pathways at 25 °C. (A) Comparison of total 22G-RNAs at 20 °C and 25 °C. The p value is a Wilcoxon unpaired test (two tailed). (B) Comparison of miRNA reads at 20 °C and 25 °C. The p value is a Wilcoxon unpaired test (two tailed). (C) Change in piRNA levels between 20 °C and 25 °C normalised by the miRNA reads observed in (B). p value is a Wilcoxon unpaired test (two tailed). (D) Comparison of expression of motif-independent piRNA between 20 °C and 25 °C. p value is a Wilcoxon unpaired test (two tailed). (E) Histogram of the p value for a reduction in motif-dependent piRNAs at 25 °C relative to 20 °C over multiple samples of motif-dependent piRNAs with the same size as the number of motif-independent piRNAs detected. The p value is a Wilcoxon-unpaired test for a reduction (one tailed). (F) Histogram reporting the p value for the change in motif-dependent piRNAs at 25 °C relative to 20 °C being larger than the change in motif-independent piRNAs over multiple samples of motif-dependent piRNAs with the same size as the number of motif-independent piRNAs detected. The p value is a Wilcoxon-unpaired test for a larger difference in motif-dependent piRNAs (one tailed). (PDF 2121 kb