Additional file 9: of Dynamics of microbial populations mediating biogeochemical cycling in a freshwater lake

Control analysis of Mystic Lake and control samples. a.) PCoA plot of samples colored according to depth name, with surface and reagent negative control samples in blue, and environmental samples in red with Bray-Curtis as distance metric. This demonstrates that negative samples are distinct from environmental samples. b.) Comparison between the observed read count and input template concentration for OTU sequences matching the mock community sequences (primer site exact matches only) for one representative mock community. This demonstrates that input template concentration largely explains the resulting read count distribution. c.) PCoA plot of samples colored according to process group (various colors) with Bray-Curtis as distance metric. This demonstrates that sample do not cluster according to process group. d.) Comparison of the average and standard deviation of Bray-Curtis distance metric for technical and biological replicates as compared to the average and standard deviation of all samples. This demonstrates that most of the variability in the dataset is not due to methodological errors. (TIF 1362Â kb