Nrf2 silencing reduces GSTA2 but not HO-1 mRNA levels.

(A) Knockdown of Nrf2 with siNrf2 in control and HT-treated (200 μM) normoxic and hypoxic cells. The results are expressed as mRNA level relative to normoxic scr siRNA/non HT-treated cells after normalization against PPIA. (B) Effect of Nrf2-silencing on the transcription level of GSTA2 and HO-1 in normoxic and hypoxic cells after treatment with HT 200 μM. The results are expressed as mRNA levels relative to normoxic HT-treated cells after normalization against PPIA. Values represent the mean ± SD from three independent experiments. Statistically significant differences with the corresponding non-treated normoxic cells: * p# p## pa pb p<0.05.