Shh and Fgf signaling in <i>Lrp4</i> and <i>Wise</i> mutants during palatal rugae development.

(A, B, Q, R) Sagittal sections of developing palate between midline and molar region showing in situ hybridization of Gli1 (A, B) and Erm (Q, R) in wild-type (A, Q), Shh-K14 (B) mice and Lrp4 mutant mice (R) at E13.5. Arrowheads indicating Erm expression in developing palatal rugae. (A) Box showing large magnification images of developing rugae region. (C, D) SEM image of palatal rugae of wild-type (C) and Shh-K14 (D) mice at birth. Arrowheads indicating palatal rugae presumptive region. (E-P) Whole mount explants showing Shh (E-N) and Erm (O, P) transcription in palatal rugae of wild-type (E, G, I, K, M, O), Lrp4 (N) and Wise (F, H, J, L, P) mutant mice at E12.5 (G, H), 13.5 (I, J, O, P), E14.0 (K, L) and E14.5 (E, F, M, N). Scale bar; 200μm.