Melting curve analyzes of the 10 reference candidate genes evaluated in this study.</p
BACKGROUND: High-resolution melting curve analysis is an accuratemethod formutation detection in gen...
Expression stability of 12 candidate reference genes analyzed by Norm Finder (M).</p
<p>For simplification in the figure we show only the standard curves relative to 0% DNA methylation ...
<p>A. Melting curve constructed using WT allele-specific primers. B. Melting curve constructed using...
<p>Standard curves were generated with a 10-fold dilution for each reference gene (A, D, G, J, M, P)...
<p>a1and a2 represent the melt curve and peak of β-actin gene, respectively; b1 and b2 represent the...
<p>Results from standard curves of the selected candidate references genes: slopes, amplification ef...
<p>Melting curves of <i>APC</i> gene(A): the standards and a sample in duplicate (highlighted); Melt...
Target and reference genes, primers sequences and melting temperatures for qPCR analysis.</p
<p>HRM analysis starts with a PCR amplification of the amelogenin gen. The amplification is in the p...
<p>Blue curves show the rate of change of fluorescence as a function of temperature. Red bars indica...
<p>All of the amplicons showed a single peak in melting curve analysis. After qPCR cycles, a melting...
Melting curve (fluorescence versus temperature) of blaSHV gene amplification products.</p
<p>(A) Original melting curves. (B) Melting curves after logarithm calculations. Blue, green, and re...
<p>Results show typical melt curves for wild type and single null mutants for the A, B and D genomes...
BACKGROUND: High-resolution melting curve analysis is an accuratemethod formutation detection in gen...
Expression stability of 12 candidate reference genes analyzed by Norm Finder (M).</p
<p>For simplification in the figure we show only the standard curves relative to 0% DNA methylation ...
<p>A. Melting curve constructed using WT allele-specific primers. B. Melting curve constructed using...
<p>Standard curves were generated with a 10-fold dilution for each reference gene (A, D, G, J, M, P)...
<p>a1and a2 represent the melt curve and peak of β-actin gene, respectively; b1 and b2 represent the...
<p>Results from standard curves of the selected candidate references genes: slopes, amplification ef...
<p>Melting curves of <i>APC</i> gene(A): the standards and a sample in duplicate (highlighted); Melt...
Target and reference genes, primers sequences and melting temperatures for qPCR analysis.</p
<p>HRM analysis starts with a PCR amplification of the amelogenin gen. The amplification is in the p...
<p>Blue curves show the rate of change of fluorescence as a function of temperature. Red bars indica...
<p>All of the amplicons showed a single peak in melting curve analysis. After qPCR cycles, a melting...
Melting curve (fluorescence versus temperature) of blaSHV gene amplification products.</p
<p>(A) Original melting curves. (B) Melting curves after logarithm calculations. Blue, green, and re...
<p>Results show typical melt curves for wild type and single null mutants for the A, B and D genomes...
BACKGROUND: High-resolution melting curve analysis is an accuratemethod formutation detection in gen...
Expression stability of 12 candidate reference genes analyzed by Norm Finder (M).</p
<p>For simplification in the figure we show only the standard curves relative to 0% DNA methylation ...