Changes in cancer-HBME total adhesion force with antibody treatment.

In adhesion between MB435 and HBMEC-60 (A), from 0.5 to 30 to 60 sec contact times, total adhesion forces were significantly reduced by antibodies to TF-Ag and β1 showing descending inhibition dynamics with the highest inhibition at 0.5 sec, compared to the baseline; while anti-α3 and anti-Gal-3 showed ascending inhibition dynamics with the highest inhibition at 60 sec. As positive control, activating β1 antibody (Activ β1) induced the greatest adhesion (64.55%) at 0.5 sec, which is in agreement with what was observed with inhibiting anti-β1 antibody. In adhesion between MB231 and HBMEC-60 (B), significant inhibition was observed at the 60 sec contact time with antibodies against TF-Ag (-23.26%), β1 (-23.81%) and Gal-3 (-41.71%), but not statistically significant with anti-α3, compared to the baseline. *p 6.