<i>W</i>. <i>mellicola</i> colonization of Altered Schaedler Flora (ASF) mice does not cause changes to ASF bacterial communities.

(A) Total fungal burden was measured in fecal samples from the indicated animals (day 6 after fungal gavage as indicated) by quantitative PCR (n = 3–5 mice per group). Results reported as relative abundance of fungal DNA per stool weight. (B) Fecal W. mellicola levels were measured by quantitative rDNA PCR normalized to stool weight. SPF column depicts unmodified mice from our facility with native W. mellicola colonization. (C) Total bacterial abundance in fecal samples was assessed by quantitative PCR of 16S rDNA. Results reported as relative abundance of bacterial DNA per stool weight. (D) Heat map of relative abundance of the 8 ASF bacterial species in Wallemia-colonized ASF mice compared to control ASF mice. Figures are a representative example of experiment that was independently performed two times except panel D which depicts combined result of experiment that was independently performed three times. Statistical comparisons in panel A *, p 0.05).