Commensal fungi examined in this manuscript are also detectable in the human gastrointestinal system.

(A) Fungal DNA content per stool weight from healthy human volunteers with no recent antibiotic use. Levels of the indicated fungi were assessed by quantitative PCR of rDNA (by comparison to standard curves generated from control samples with defined fungal DNA content). (B) Wallemia abundance among the Wallemia-colonized humans is similar to unmodified SPF mice from our facility but less than mice with Wallemia-enhanced colonization. (C) Total fungal DNA burden in healthy human volunteers by rtPCR per stool weight. Red dots indicate human samples with detectable Wallemia. Wallemia-colonized humans do not generally have higher total fungal burden in this cohort compared to individuals without Wallemia colonization.