HSV-1 infection induces the accumulation of HIRA complex proteins in PML NBs and co-localization with latent/quiescent HSV-1 genomes in vDCP NBs.

Immuno-FISH performed in BJ cells not infected (ni) (i, iii, v, vii) or infected for 2 days (ii, iv, vi, viii) with in1374. HIRA (i and ii), UBN1 (iii and vi), CABIN1 (v and vi), ASF1a (vii and viii) (gray, green), HSV-1 genomes (gray, red), and PML (gray, blue) were detected. Arrows indicate examples of the detection of HIRA complex proteins in vDCP NBs. Scale bars = 5 μm.