MOESM1 of Functional intron-derived miRNAs and host-gene expression in plants

Additional file 1: Figure S1. Nucleotide sequences of all ff-gLUC/aimiRNA constructs used in this study. gatatc: − EcoR V site; gaattc: EcoR I site; capital letter: exon sequences; small letters black: intron sequences; small letters blue: Id-amiRNA sequences based on miR319a. Specific sequences targeting TCP, LUC or PHYB are underlined. 5′intron splice sequences are boxed (ag: 3′intron splice site, gt: 5′intron splice site). Figure S2. RT-PCR products sequence from ffgLUCdelaimiR-319a transgenic plant. Figure S3. A similar transformation efficiency were confirmed by quantify the DsRED gene expression in transient assay samples. The quantification data is normalized against the N. benthamiana reference with UBI3 as internal control. Error bars represent standard error. Figure S4. Confirmation of mature aimiR-319a expression in stem-loop RT-PCR assay. Illustration of RT-PCR method for amplification of mature microRNA (A). Gel electrophoresis results from stem-loop RT (B). ffgLUC plant used as a positive control for endogenous mature miR319a. A mature microRNA specific forward primer and universal reverse primer were used for PCR amplification. Mature microRNA products were obtained using 25 cycling of RT-PCR and analysed on 4% agarose gel in 1xTAE. Predicted products were compared with 50 bp DNA ladder. Figure S5. PHYB silencing phenotype under constant cR. Table S1. Stable transformation of Arabidopsis WT or Arabidopsis line ff-gLUC-1 with the different expression constructs. NA not applicable, *very low LUC activity; **low LUC activity. Table S2. List of primers were used in this work