Delayed wound healing in DKO mice.

A. Representative WT and DKO mice on Day 11 following the creation of bilateral, vertical, 8-mm wounds. The initial wounds were located as indicated by the arrows in the WT panel, with aberrant healing in the DKO apparent B. Quantitation of the day 11 skin surface wound area (n = 6 mice and 12 wounds per group). C. H&E stained cross-sections of skin from WT and DKO mice at day 11 after wounding with wound margins indicated by dashed lines. These skin tissue of the wounded area was collected [dotted black box, A and C (enlarged)] and sectioned perpendicular to the length on the wound (white stipple) to determine distance between hair folicles on either side of the incision. D. Quantitation of the distance between healthy hair follicles adjacent to the wound (n = 2–3 sections per wound; 12 wounds per group). Data are means ± SEM, analyzed by Student’s t test. *** p < 0.001.