miR-2909 induced cellular ultrastructural traits.

(A-B) Representative images of electron microscopy (TEM) of human PBMCs transfected with null vector containing scrambled sequence compared with miR-2909 expression vector. Globular shaped mitochondria (M) coupled with cristae-poor morphology in human PBMCs transfected with miR-2909 expression vector in contrast to normal elongated mitochondrial morphology with well aligned lamellar cristae in human PBMCs transfected with control null vector containing scrambled sequence. (C-D) Increased formation of intracellular lipid inclusion bodies (LI) in human PBMCs transfected with miR-2909 expression vector compared with control null vector containing scrambled sequence transfected human PBMCs. Scale 800nm;Final Magnification 14110X. (E-F) 50cells/case was studied under TEM for the observation of apoptotic changes in human PBMCs transfected with control vector and miR-2909 expression vector after 48h incubation period. We observed that few cells under TEM were indicating early apoptotic changes and formation of phagolysosome (PL) in human PBMCs transfected with control vector however no apoptotic changes were observed in healthy PBMCs transfected with miR-2909 expression vector. Stain: Uranyl acetate and lead citrate.