KDACi reduces cytokine-induced expression of miR-146a-5p in human islets.

(A) Human islets from three individual necro-donors were treated with or without 500 nM Givinostat (Giv) 1 h prior to addition of 300 pg/ml IL-1β, 10 ng/ml IFN-γ, and 10 ng/ml TNF-α (Mix) or no cytokines (Ctrl) for 24 h. miR-146a-5p expression was measured with qRT-PCR and normalized to the geometric mean of the housekeeping miRs miR-103a and miR-423. Data are presented as mean ± SEM of 2-ΔCT. *p<0.05 (95% confidence interval = -0.04836 to -0.004574), ***p<0.001 (95% confidence interval = 0.004108 to 0.005359). (B) Human islets from nine individual necro-donors were left untreated (control) or treated for 48 h with a mixture of 5000 U/ml TNF-α, 750 U/ml IFN-γ, and 75 U/ml IL-1β (Mix). miR-146a-5p expression was analyzed by qRT-PCR and presented as ΔCT values after normalizing to let-7c. *p<0.05.