Large scale changes in the transcriptome of <i>Eisenia fetida</i> during regeneration - Fig 5

(A) Nerve Growth Factor gene is induced in regenerated tissue (solid black line) at 15dpa, 20dpa and 30dpa. Adjusted pval is mentioned at each point. The gene is also induced in the control tissue but was not statistically significant (dashed line). Stippled line shows the fold change in the posterior (P0) to the control tissue (0C) at the time of injury (0dpa). (B) Neurofilament (NF70) genes are unregulated during regeneration in E. fetida. Expression level of various isoforms of NF70 at 15, 20 and 30 days post amputation in the regenerated tissue (15R, 20R, 30R) compared to adjacent control tissue (15C, 20C, 30C). (C) Hematoxylin and Eosin (H&E) staining of cross-section of regenerating earthworm Eisenia fetida 20 dpa. (D) Schematic figure of NGF consisting of the signal peptide, pre-NGF and NGF separated by a conserved Furin cleavage site (sequence details in S5) with phylogenetic analysis shows that the Eisenia fetida does not resemble other invertebrate NGFs like the mollusc NGF in primary protein sequence. (E-F) The signal peptide and the Pro region show minimal homology but the conserved cysteines in the mature NGF reveal structural homology to mouseNGF (mmNGF).