Growth curves for E10 (A), BxPC3 (B), and H3 (C) cell lines fully adapted to the different culture media.

Cells were either grown in conventional media with 10% FBS (blue line), conventional media with 1% FBS (red line), conventional media with 1% exosome depleted FBS (green line, E10 only as the BxPC3 and H3 cell lines were unable to proliferate in these conditions), Advanced media with 1% FBS (purple line), or Advanced media with 1% exosome depleted FBS (black line). The best growth media with the least vesicles contamination was Advanced media supplemented with 1% exosome depleted FBS (black line).