Amplification curves and anneal derivative melting temperatures for <i>aprV2</i>. gDNA dilutions and <i>aprB2</i> gDNA dilutions from 5–0.025 ng μL^-1.

Amplification curve thresholds for time to positive are indicated in red on graphs A, B and C. A–aprV2 gDNA dilutions from 5–0.025 ng μL-1 amplification; B—aprB2 gDNA dilutions from 5–0.025 ng μL-1 amplification; C–both gDNA dilutions from 5–0.025 ng μL-1 amplification. The fluorescent derivative annealing curve for the corresponding aprV2 gDNA dilutions (D), aprB2 gDNA dilutions (E), and both (F) are shown with the threshold for peak detection in the anneal derivative and the acceptable range of Tm shown in red. AprV2 controls are indicated by red and labelled ‘V’, with final concentrations (ng μL-1) of gDNA shown numerically following. AprB2 controls are indicated by blue and labelled ‘B’, with final concentrations (ng μL-1) of gDNA shown numerically following. For all, solid lines are the 1st repeat, dashed the 2nd.