Novel histone deacetylase inhibitors and embryo aggregation enhance cloned embryo development and ES cell derivation in pigs - Fig 5

Panel A: Formation of ntES cell colonies derived from the blastocyst of the 3× cloned embryos. (a-c) Morphologies of non-aggregated, 3× aggregated blastocysts (day 7) and nuclear staining of 3× blastocysts by Hoechst 33342. (d and e) embryonal outgrowths with clear ICM cells (red arrow) after culture for 8–10 days. (f, g) Typical morphology of a porcine ntES cell colony (nt79-2) after passage. (h, i) Differentiated cells after passage. Panel B: G-banding karyotypes of porcine ntES-79-2 cells at passage 11. These cells have 18 pairs of autosomes and normal XX karyotypes. Scale bar = 100 μm.