RPT-6 is required for activation of immune genes during infection.

A. Control or RNAi treated PF55G11.2::gfp animals were grown on E. coli OP50 for 12 hours. B. Fluorescence images were quantified using ImageJ software. Bars represent means ± SEM; n = 3 (t-test, ***PC. qRT-PCR analysis of immune genes in elt-2(RNAi) or rpt-6(RNAi) animals exposed to P. aeruginosa for 12 hours relative to control animals exposed to E. coli. Red bars correspond to gene expression in control animals exposed to P. aeruginosa for 12 hours relative to control animals exposed to E. coli. Error bars indicate means ± SEM; n = 3 (t-test *PD. Control, elt-2(RNAi), and rpt-6(RNAi) animals were exposed to P. aeruginosa and scored for survival, ***PE. qRT-PCR analysis of recovery genes elt-2(RNAi) or rpt-6(RNAi) animals exposed to P. aeruginosa and then recovered on E. coli plus streptomycin, relative to control animals on E. coli followed by E. coli plus streptomycin. Red bars correspond to gene expression in control animals exposed to P. aeruginosa and then recovered on E. coli plus streptomycin, relative to control animals on E. coli followed by E. coli plus streptomycin. Error bars indicate means ± SEM; n = 3 (t-test ***PF. Control, elt-2(RNAi), and rpt-6(RNAi) animals were exposed to E. coli (Ec) or P. aeruginosa (Pa) for 12 hours, treated with streptomycin, and then transferred to E. coli plus Streptomycin plates and scored for survival. Scoring started 24 hours post initial exposure to E. coli or P. aeruginosa, ***P<0.0001.