Cells from either WT virus or ΔvIL-6 tumors were stained with antibodies specific for human B cells markers and analyzed by flow cytometry. The gating strategy is indicated by brackets, where [CD45] indicates that only human CD45+ cells were analyzed for the secondary marker shown below. (A) Representative histograms of some markers that differed by virus strain. (B) Summary of mean populations that were positive for the given marker. (C) Summary of mean fluorescence intensities. P values are indicated. n = 5 WT; n = 6 ΔvIL-6.</p
<p>A) Overlay of flow cytometry and array data on surface markers used for sorting of the bone marro...
<p>Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populat...
<p>A. Flow cytometry analysis for the expression of CD24 and CD44 in treated cells. B. Real time PCR...
BJAB cells containing either WT virus or the ΔvIL-6 strain were stained with antibodies specific for...
BJAB WT cells from culture and single cell suspensions from solid tumors were stained for common B c...
Example of the gating strategy used to characterize the immune cells subsets present in analyzed tum...
Cells collected from ascites were stained with a 9-color flow cytometry panel and analyzed in an X-2...
<p>Following exclusion of dead cells and debris, two different gating strategies were employed to ev...
<p>Representative analyses from a patient with Tb (A) and an LTBI (B) are shown. Open red circles an...
<p>Age-matched sun-exposed skin or tumour lesions were excised and digested as outlined in <i>Materi...
Illustrative example of the gating strategy employed for the analysis of flow cytometry data. BSR-T7...
<p>Cultured tumor cells (SK-BR-3 (breast), PC-9 (non-small cell lung), PC-14 (non-small cell lung), ...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
A precise identification and phenotypic characterization of human B-cell subsets is of crucial impor...
<p>A) Overlay of flow cytometry and array data on surface markers used for sorting of the bone marro...
<p>Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populat...
<p>A. Flow cytometry analysis for the expression of CD24 and CD44 in treated cells. B. Real time PCR...
BJAB cells containing either WT virus or the ΔvIL-6 strain were stained with antibodies specific for...
BJAB WT cells from culture and single cell suspensions from solid tumors were stained for common B c...
Example of the gating strategy used to characterize the immune cells subsets present in analyzed tum...
Cells collected from ascites were stained with a 9-color flow cytometry panel and analyzed in an X-2...
<p>Following exclusion of dead cells and debris, two different gating strategies were employed to ev...
<p>Representative analyses from a patient with Tb (A) and an LTBI (B) are shown. Open red circles an...
<p>Age-matched sun-exposed skin or tumour lesions were excised and digested as outlined in <i>Materi...
Illustrative example of the gating strategy employed for the analysis of flow cytometry data. BSR-T7...
<p>Cultured tumor cells (SK-BR-3 (breast), PC-9 (non-small cell lung), PC-14 (non-small cell lung), ...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
A precise identification and phenotypic characterization of human B-cell subsets is of crucial impor...
<p>A) Overlay of flow cytometry and array data on surface markers used for sorting of the bone marro...
<p>Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populat...
<p>A. Flow cytometry analysis for the expression of CD24 and CD44 in treated cells. B. Real time PCR...