MOESM5 of Gene expression analysis of potential morphogen signalling modifying factors in Panarthropoda

Additional file 5: Fig. S3. Confocal microscopy of Ek-sFRP125. Stage 13 embryo. Anterior to the left, dorsal up. The embryo is stained with FastRed (for sFRP125) and DAPI (for DNA). Optical sections were taken every 6.5 μm. A Z-stack. The arrow points to signal in L13 (mesodermal). The arrowhead points to signal anterior in L6 (ectodermal). In all panels, arrow and arrowhead point to identical position. A′ Optical section z-08. Strong signal in L13, no signal in L6. A´´ Optical section z-14. Disappearing signal in L13 (quenched by overlying DAPI signal (ectodermal cells)). Appearing signal in L6 (no overlying DAPI signal). A′′′ Optical section z-25. Signal in L13 disappeared. Strong DAPI signal (ectodermal cells). Strong signal in L6. Abbreviations: fap, frontal appendage; j, jaw; L, leg; sp, slime papilla