Relationship between DENV serotype, clinical disease severity, viraemia and T cell responses.

A: Ex vivo ELISpot responses to DENV NS1, NS3, NS5 and combined DENV-ALL overlapping peptides in patients who were infected with DENV-1 (n = 30), DENV-2 (n = 19) or who were aviraemic (n = 19). Error bars represent the median and the interquartile range. **P<0.05, **P<0.01, ***P<0.001. (B) Correlation between DENV NS3-specific T cell responses and degree of viraemia (Spearman’s r = -0.47, p = 0.0003). (C) Ex vivo ELISpot responses to DENV NS1, NS3, NS5 and the overall DENV overlapping peptides in patients who had primary dengue (P) (n = 19) and secondary dengue infection (S) (n = 48).