Western blot analysis of BCL-XL and GAPDH in J/Neo and J/BCL-XL cells (A), and flow cytometric analysis of the effect of CMEP-NQ on NOC-, 2-MeO-E₂-, and CPT-induced alterations in cell cycle distribution (B and C) and Δψm loss (D and E) in Jurkat T cell clone transfected with a BCL-XL-expression vector (J/BCL-XL cells).

After pretreatment with 7.5 μM CMEP-NQ for 1 h, J/BCL-XL cells (5 × 105/ml) were treated with 0.3 μM NOC, 1.0 μM 2-MeO-E2, or 0.02 μM CPT for 17 h and then subjected to flow cytometric analysis of cell cycle distribution, Δψm loss, and BAK activation as described in the Materials and Methods. Western blot and flow cytometric analyses were performed as described in the Materials and Methods. A representative result is shown; two additional experiments yielded similar results. Error bars represent standard deviations.