Effect of growth and lineage diversity in pre-stasis HMEC in M87A ± fetal bovine serum.

(A) Curves showing PD as a function of time in days for 240L grown in M87A with and without 0.25% FBS from 3p. Growth rates were only assessed to 10p, thus the curves to not reflect cultures reaching stasis. Pseudocolor dot plots representing flow cytometry measurements of CD227 and CD10 expression in 4p HMEC cultured in (B) regular M87A media or (C) M87A without fetal bovine serum (which is normally at 0.25%). Whereas growth rates are identical with or without serum, LEP cells are lost more rapidly in serum-free M87A.