Additional file 2: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

Publication date

October 2018

DOI

10.6084/m9.figshare.7171301.v1

Abstract

Chemicals and antibodies; Plasmid and lentiviral constructs; Immunoprecipitation and Western blotting; qPCR; ChIP assay; Immunofluorescence. (DOCX 22 kb

Extracted data

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Data Protection

Additional file 2: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

Open link

Publication date

October 2018

DOI

10.6084/m9.figshare.7171301.v1

Abstract

Chemicals and antibodies; Plasmid and lentiviral constructs; Immunoprecipitation and Western blotting; qPCR; ChIP assay; Immunofluorescence. (DOCX 22 kb

Extracted data

Victor Martinez (2539555)
Imelda Ontoria-Oviedo (798472)
Carolina Ricardo (4480828)
Sian Harding (4480822)
Rosa Sacedon (4480825)
Alberto Varas (760278)
Agustin Zapata (370545)
Pilar Sepulveda (3633409)
Angeles Vicente (760277)

September 2017

Detailed methods: lentiviral production and transduction, metabolic assays, and list of reagents for...

Additional file 1: of Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo

L. Mendes (2034364)
H. Katagiri (4874164)
W. Tam (4874173)
Y. Chai (4874170)
L. Geris (4874167)
S. Roberts (2839985)
F. Luyten (4874161)

February 2018

Table S1. Details of the antibodies and immunohistochemistry protocols employed. (DOCX 17 kb

Additional file 1: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Table S1. Main characteristics of the US Patients and Normal donors cohort. Table S2. Main character...

Additional file 5: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S3. MM Gfi1 o/e cells produce higher levels of osteoclastogenic factors. MM.1S EV and Gfi1 o/...

Additional file 4: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S2. Gfi1 overexpression increases metabolic activity and confers protection from Btz-induced ...

Additional file 3: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S1. Gfi1-KD induces apoptosis in MM cells regardless of their p53 status. MM.1S cells, lentiv...

Additional file 1: of Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis

Stefania Raimondo (145104)
Laura Saieva (3762613)
Emanuela Vicario (6187787)
Marzia Pucci (6187790)
Denise Toscani (5821787)
Mauro Manno (179184)
Samuele Raccosta (430584)
Nicola Giuliani (5821811)
Riccardo Alessandro (145120)

January 2019

Figure S1. Evaluation by quantitative Real Time PCR of mRNA expression of AREG in HMCLs (A) and in M...

Additional file 1: of Myeloma-derived macrophage inhibitory factor regulates bone marrow stromal cell-derived IL-6 via c-MYC

Rachel Piddock (5229290)
Christopher Marlein (5229296)
Amina Abdul-Aziz (5229281)
Manar Shafat (5229287)
Martin Auger (5229278)
Kristian Bowles (5229284)
Stuart Rushworth (5229293)

May 2018

Figure S1. (A) Relative transcriptional levels of MIF expression in B cells, T cells, non-malignant ...

Additional file 1: of Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor

Chenyue Ding (629020)
Qinyan Zou (4927786)
Fuxin Wang (1512043)
Huihua Wu (4282834)
Rulei Chen (4282843)
Jinghuan Lv (4282840)
Mingfa Ling (4927783)
Jian Sun (1234941)
Wei Wang (17594)
Hong Li (20183)
Boxian Huang (4282837)

March 2018

Figure S1. An antibody microarray to test the level of the growth factors derived from the hAMSCs. (...

Additional file 1: of A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture

Basem Abdallah (3581783)
Abdullah Alzahrani (3372671)
Ashraf Abdel-Moneim (6286544)
Nicholas Ditzel (127179)
Moustapha Kassem (101848)

February 2019

Table S1. List of primers used for qRT-PCR. Table S2. Full osteogenic gene expression list (total 84...

Additional file 2: of Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis

Stefania Raimondo (145104)
Laura Saieva (3762613)
Emanuela Vicario (6187787)
Marzia Pucci (6187790)
Denise Toscani (5821787)
Mauro Manno (179184)
Samuele Raccosta (430584)
Nicola Giuliani (5821811)
Riccardo Alessandro (145120)

January 2019

Figure S2. Schematic representation of the role of MM-exosomes in bone microenvironment. (TIF 3372 k...

Additional file 4: of TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma

Hua Bai (145775)
Huayuan Zhu (5863823)
Qing Yan (72879)
Xuxing Shen (5863826)
Xiupan Lu (5863829)
Juejin Wang (5863832)
Jianyong Li (133044)
Lijuan Chen (150349)

October 2018

Figure S4. a Double-staining Immunofluorescence detection showing TRPV2 (red) and CD38 (green) in A5...

Additional file 2: of TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma

Hua Bai (145775)
Huayuan Zhu (5863823)
Qing Yan (72879)
Xuxing Shen (5863826)
Xiupan Lu (5863829)
Juejin Wang (5863832)
Jianyong Li (133044)
Lijuan Chen (150349)

October 2018

Figure S2. a-j Point graph depicting the levels of TRPV2â6 mRNA in MM patients BM plasma cells fro...

Additional file 1: of Overexpression of hypoxia-inducible factor 1 alpha improves immunomodulation by dental mesenchymal stem cells

Victor Martinez (2539555)
Imelda Ontoria-Oviedo (798472)
Carolina Ricardo (4480828)
Sian Harding (4480822)
Rosa Sacedon (4480825)
Alberto Varas (760278)
Agustin Zapata (370545)
Pilar Sepulveda (3633409)
Angeles Vicente (760277)

September 2017

Detailed methods: lentiviral production and transduction, metabolic assays, and list of reagents for...

L. Mendes (2034364)
H. Katagiri (4874164)
W. Tam (4874173)
Y. Chai (4874170)
L. Geris (4874167)
S. Roberts (2839985)
F. Luyten (4874161)

February 2018

Table S1. Details of the antibodies and immunohistochemistry protocols employed. (DOCX 17 kb

Additional file 1: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Table S1. Main characteristics of the US Patients and Normal donors cohort. Table S2. Main character...

Additional file 5: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S3. MM Gfi1 o/e cells produce higher levels of osteoclastogenic factors. MM.1S EV and Gfi1 o/...

Additional file 4: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S2. Gfi1 overexpression increases metabolic activity and confers protection from Btz-induced ...

Additional file 3: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Daniela Petrusca (5821784)
Denise Toscani (5821787)
Feng-Ming Wang (5821790)
Cheolkyu Park (5821793)
Colin Crean (5821796)
Judith Anderson (5821799)
Silvia Marino (197193)
Khalid Mohammad (5642813)
Dan Zhou (29408)
Rebecca Silbermann (5821802)
Quanhong Sun (141209)
Noriyoshi Kurihara (5821805)
Deborah Galson (5821808)
Nicola Giuliani (5821811)
G Roodman (5821814)

October 2018

Figure S1. Gfi1-KD induces apoptosis in MM cells regardless of their p53 status. MM.1S cells, lentiv...

Additional file 1: of Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis

Stefania Raimondo (145104)
Laura Saieva (3762613)
Emanuela Vicario (6187787)
Marzia Pucci (6187790)
Denise Toscani (5821787)
Mauro Manno (179184)
Samuele Raccosta (430584)
Nicola Giuliani (5821811)
Riccardo Alessandro (145120)

January 2019

Figure S1. Evaluation by quantitative Real Time PCR of mRNA expression of AREG in HMCLs (A) and in M...

Additional file 1: of Myeloma-derived macrophage inhibitory factor regulates bone marrow stromal cell-derived IL-6 via c-MYC

Rachel Piddock (5229290)
Christopher Marlein (5229296)
Amina Abdul-Aziz (5229281)
Manar Shafat (5229287)
Martin Auger (5229278)
Kristian Bowles (5229284)
Stuart Rushworth (5229293)

May 2018

Figure S1. (A) Relative transcriptional levels of MIF expression in B cells, T cells, non-malignant ...

Additional file 1: of Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor

Chenyue Ding (629020)
Qinyan Zou (4927786)
Fuxin Wang (1512043)
Huihua Wu (4282834)
Rulei Chen (4282843)
Jinghuan Lv (4282840)
Mingfa Ling (4927783)
Jian Sun (1234941)
Wei Wang (17594)
Hong Li (20183)
Boxian Huang (4282837)

March 2018

Figure S1. An antibody microarray to test the level of the growth factors derived from the hAMSCs. (...

Basem Abdallah (3581783)
Abdullah Alzahrani (3372671)
Ashraf Abdel-Moneim (6286544)
Nicholas Ditzel (127179)
Moustapha Kassem (101848)

February 2019

Table S1. List of primers used for qRT-PCR. Table S2. Full osteogenic gene expression list (total 84...

Additional file 2: of Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis

Stefania Raimondo (145104)
Laura Saieva (3762613)
Emanuela Vicario (6187787)
Marzia Pucci (6187790)
Denise Toscani (5821787)
Mauro Manno (179184)
Samuele Raccosta (430584)
Nicola Giuliani (5821811)
Riccardo Alessandro (145120)

January 2019

Figure S2. Schematic representation of the role of MM-exosomes in bone microenvironment. (TIF 3372 k...

Additional file 4: of TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma

Hua Bai (145775)
Huayuan Zhu (5863823)
Qing Yan (72879)
Xuxing Shen (5863826)
Xiupan Lu (5863829)
Juejin Wang (5863832)
Jianyong Li (133044)
Lijuan Chen (150349)

October 2018

Figure S4. a Double-staining Immunofluorescence detection showing TRPV2 (red) and CD38 (green) in A5...

Additional file 2: of TRPV2-induced Ca2+-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma

Hua Bai (145775)
Huayuan Zhu (5863823)
Qing Yan (72879)
Xuxing Shen (5863826)
Xiupan Lu (5863829)
Juejin Wang (5863832)
Jianyong Li (133044)
Lijuan Chen (150349)

October 2018

Figure S2. a-j Point graph depicting the levels of TRPV2â6 mRNA in MM patients BM plasma cells fro...

Additional file 1: of Overexpression of hypoxia-inducible factor 1 alpha improves immunomodulation by dental mesenchymal stem cells

Victor Martinez (2539555)
Imelda Ontoria-Oviedo (798472)
Carolina Ricardo (4480828)
Sian Harding (4480822)
Rosa Sacedon (4480825)
Alberto Varas (760278)
Agustin Zapata (370545)
Pilar Sepulveda (3633409)
Angeles Vicente (760277)

September 2017

Detailed methods: lentiviral production and transduction, metabolic assays, and list of reagents for...

L. Mendes (2034364)
H. Katagiri (4874164)
W. Tam (4874173)
Y. Chai (4874170)
L. Geris (4874167)
S. Roberts (2839985)
F. Luyten (4874161)

February 2018

Table S1. Details of the antibodies and immunohistochemistry protocols employed. (DOCX 17 kb

Additional file 2: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Abstract

Extracted data

Additional file 2: of Growth factor independence 1 expression in myeloma cells enhances their growth, survival, and osteoclastogenesis

Abstract

Extracted data

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