Outline of cloning procedure using the R4pL1MpGWB system.
- Shoji Mano (140137)
- Ryuichi Nishihama (746387)
- Sakiko Ishida (799922)
- Kazumi Hikino (3142617)
- Maki Kondo (52973)
- Mikio Nishimura (52980)
- Katsuyuki T. Yamato (586838)
- Takayuki Kohchi (746388)
- Tsuyoshi Nakagawa (574808)
Publication date
October 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Promoter entry clones are constructed by a BP reaction between pDONR P4-P1R and an attB4-promoter-attB1 fragment. The promoter entry clones and R4L1pMpGWB vectors are used for a bipartite LR reaction. Note: as pDONR P4-P1R has been discontinued, pENTR 5′-TOPO (Thermo Fisher Scientific) can be used as an alternative for promoter entry clone production. Arrowheads, T-DNA border sequences; B1, attB1; B4, attB4; P4, attP4; P1R, attP1R; L1, attL1; L4, attL4; R1, attR1; R4, attR4; Kmr, kanamycin-resistant marker; Cmr, chloramphenicol-resistant marker; Specr, spectinomycin-resistant marker; ccdB, negative selection marker used in bacteria.</p
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