Increased proliferation of activated H564 B cells and depositions of anti-ssRNA antibody and C3 in the kidneys of MFG-E8-/- mice.

A: LPS activated H564 B cells were labeled with CFSE before transferring into w.t. and MFG-E8-/- hosts. Three days later, the proliferation of transferred cells in the spleen was quantified by CFSE dilution. The result is representative of two experiments. B: Two weeks after the transfer of activated H564 B cells, depositions of auto-antibody and C3 in the recipients’ kidney were determined by immunofluorescence staining using antibodies specific for the idiotype and C3, respectively. The staining intensities were quantified by Image J. Each data point represents the average of 5 glomeruli from an individual animal.