Bovine <i>OCT4</i> distal enhancer silenced in primed pluripotent stem cells.

A: The parental bOCT4-DE-GFP+ ESCs and their EpiSC colonies formed after subculturing (P4) in EpiSC medium. Bar = 120 μm (left) or 120 μm (right).B: qRT-PCR gene expression ratio for primed- and naïve-state pluripotency markers between the converted EpiSCs (p4) and their parent ESCs. n = 3.C: Left: Immunostaining of pluripotent markers in ESCs and the converted EpiSCs. Bar = 60 μm. Right: Quantification of fluorescence intensity from immunostaining. Values are arbitrary unit (AU) normalized with DAPI Stain. **: p OCT4-DE-GFP positive ESCs and their converted EpiSCs (p4). GFP intensity represents mean±sd, n = 3.F: bOCT4-DE-GFP negative EpiSC colonies (left) were further expanded and developed into GFP+ ESC-like colonies (right) upon culture in 2i/LIF medium and passaged for 3 times (P3). Bar = 250 μm.