Treatment of CSE modulated miR-133a-3p in HPV(+) cells.

(A) UMSCC47 cells were treated with different concentrations (0, 5, 10, 20%) of CSE for eight days and tested for relative expression of miRNAs. * denotes p-value of (B) Estimation of miR-133a-3p target mRNAs. UMSCC47 cells were treated with 10% CSE for eight days and tested for indicated mRNA levels by qRT-PCR. Actin and RPS18 genes were used as endogenous controls. (C) Western blot analysis of EGFR and HuR in UMSCC47 cells after eight days treatment with 10% CSE. β-Actin was used as loading control. (D) Graphical representation of quantitative levels of HuR and EGFR protein levels. β-Actin was used for normalization. (E) Relative mRNA levels of EGFR and HuR in smokers (n = 9) and non-smokers (n = 13) tissue samples, as obtained by qRT-PCR. Actin and RPS18 were used as endogenous controls.