Additional file 4: of Tumor-derived exosomes induce N2 polarization of neutrophils to promote gastric cancer cell migration

Figure S4. BGC-Ex-treated neutrophils promoted gastric cancer cell proliferation and endothelial cell tube formation. A. ROS production in BGC-Ex-treated neutrophils was measured by flow cytometric analysis. B. Wright’s stain for BGC-Ex-treated neutrophils. Magnification, 200×. C. The proliferation rate of gastric cancer cells treated with supernatant from BGC-Ex-treated neutrophils was measured by CCK8 assay. D. T Tube formation assay for endothelial cells following treatment with supernatant from BGC-Ex-treated neutrophils. E. qRT-PCR assays for MMP-9, VEGF, CXCR2, and TLR4 expression in BGC-Ex-treated neutrophils. F. Western blot assays for p38 and Akt expression in neutrophils treated with BGC-Ex. **P<0.01, and *P