Rhizoplane bacterial response to different substrates.

Bacterial DGGE fingerprints based on 16S rRNA gene fragments amplified from total genomic DNA extracted from plants grown in the different substrates (+ARD, -ARD and Control), coming from different combinations of substrates: +ARD, +ARD; -ARD, -ARD; -ARD, +ARD and +ARD, Control. On the right side the respective UPGMA cluster analysis of the samples. Red arrows indicate differentiating bands detected only in the bacterial fingerprint of ARD soils. The bands were excised, reamplified, cloned and sequenced (S2 Fig). BS: Bacterial standard of 16S rRNA gene fragments from 11 bacterial strains included according to Heuer et al. [38]. Samples are enumerated with the same number in DGGE and UPGMA tree.