<i>osgst4</i> mutant exhibits defective growth and reduced tolerance to oxidative stress.

A. Schematic diagram indicating the T–DNA insertion site in genomic region in osgst4. B. Genotyping of osgst4 T2 seedlings performed via PCR analysis. C. Transcript levels of OsGST4 in wild type (DJ) and osgst4 mutant by qRT–PCR analysis. D. Seven–day–old seedlings of DJ and osgst4 mutant grown in standard 1/2 MS medium. Scale bar, 4 cm. E–G. Measurement of primary root length, lateral root number and shoot length in image D. H. Plant architecture of DJ and osgst4 mutant at mature stage. Scale bar, 10 cm. I–K. Comparison of plant height, seed setting rate and germination rate, respectively, between DJ and osgst4 mutant in image H. L and M. Quantification of H2O2 content in the shoot and root of 7–day–old seedlings in response to H2O2 or NaCl, respectively. N and O. Detached leaves from 4–week–old DJ and osgst4 plants exposed to 100 mM H2O2 or 150 mM NaCl for 3 days to indicate the oxidative stress tolerance. NBT, nitroblue tetrazolium. DAB, 3, 3’–diaminobenzidine. Three independent experiments were performed. Data are means ± SE (n = 30). The statistical significance of the measurements using one-way analysis of variance (ANOVA) was determined using Student’s t-test. Asterisks indicate the significant difference between OsMADS25 transgenic lines and WT plants (*P **P ***P < 0.001).