Pancreatic decellularized matrix (DCM) proteins maintains the expression of β-cell gene expression <i>in vitro</i>.

A) Gene expression analysis of β-cell transcription factors from dissociated rat pancreatic islets cultured under low glucose (5 mM) conditions for 4 weeks (n = 4). B) Gene expression analysis of β-cell transcription factors from whole rat pancreatic islets cultured under physiological glucose (11 mM) conditions for 4 weeks (n = 4). C) Dissociated islet cells were plated on cell culture plates coated with whole solubilized pancreatic decellularized matrix under low glucose (5 mM) conditions. Gene expression was analyzed on days 2, 10, and 24 of culture relative to non- coated controls. Whole DCM increased the expression of key β-cell transcription factors including Ins2, Mafa, Pdx1, Nkx2.2, Nkx6.1 and Glut2 (n = 4). Data presented as mean ± SEM, where p<0.05 was considered statistically significant.