NF-κB translocation of select anti-inflammatory extracts.

A: Fluorescence microscopy images of NF-κB stained HeLa-TLR4 dual reporter cells incubated with extracts or vehicle (70% ethanol), followed by stimulation with LPS-EB. For better visibility, images were cropped and adjusted in brightness and contrast. Scale bar = 50 μm. B: Quantitative evaluation of NF-κB p65 translocation. Mean fluorescence ratios of nuclear to cytoplasmic NF-κB p65 were calculated and compared to ethanol control. Data represents means ± SD (n = 3, 72 images (fields) per experiment and per treatment condition). Dunnett’s post hoc test with **p<0.01; ***p<0.001 compared to ethanol control.